Hoverfly Atlas

Summary

Hoverflies are essential pollinators enormously contributing to plant diversity and agricultural production, but they are declining. However, despite their importance for preserving both biodiversity and food security, hoverflies have been severely understudied compared to other major pollinators such as bees. This substantial gap in knowledge is even more problematic in Luxembourg, one of the most urbanized countries in the world, where future land use change for new infrastructure and urban development will likely be considerable, because of demographic growth. To protect their functional role in the provision of key ecosystem services, we need to document the diversity and distribution of hoverflies. Indeed, developing this basic knowledge about hoverflies is a necessary first step towards a better understanding of how landscape disturbance affect the dynamics of their populations and, in the end, for developing future conservation projects and red lists. Developing an atlas of hoverflies in Luxembourg is, therefore, crucial. Beyond actively hand netting and trapping specimens, we plan to use georeferenced bulk samples from the historical wet collection of the MNHN, as well as collect data from recent and ongoing projects from other institutions to complement our datasets.

Project status

  • Proposal accepted by the Ministre de l’Environnement, du Climat et du Développement durable in the context of the funding line Appel à projets « Pollinisateurs ». Duration: 57 months (04/2023 – 12/2027).
  • Host institution: Fondation faune-flore @ Centre de recherche scientifique, Musée national d’histoire naturelle.
  • Partners: MECB

Funding

With financial support from the Fonds pour la protection de l’environnement

Contacts

National Museum of Natural History (MNHNL)
Research center
Life science department
25, rue Münster
L-2160 Luxembourg

Publications

 

Beelibre.lu: Luxembourg’s open library of wild bee species profiles, pollen data, DNA barcodes and bibliographic references

Summary

Wild bees are insect pollinators of fundamental relevance for both crops and wild plants. Their ecosystem services are valued in billions of euros and their disappearance could cause irreparable damage to biodiversity, plant reproduction, and human food production. Swift action must be taken to prevent further declines on regional wild bee biodiversity, but the success of effective conservation strategies relies heavily on our understanding of wild bee taxonomy and ecology. For this purpose, well curated reference material plays a vital role.

High quality descriptions are necessary for morphological wild bee identification in monitoring programs, voucher specimens from natural history collections are important to address open research questions and for general educational purposes, and validated DNA barcodes are crucial for molecular identification of morphologically undeterminable cryptic species or complicated taxa groups.

However, reliable reference data of all kinds for the wild bee fauna of Luxembourg is usually scattered and hard to find. Identification keys often lack images of appropriate resolution or regional relevance. Data regarding regional wild bee species diversity and distribution is spread among diverse sources, languages and media formats. Moreover, despite the growing volume of DNA barcodes available in the Barcode of Life Data Systems (BOLD), misidentifications and gaps in the regional reference library constrain the detection capacity of DNA based identification tools. Finally, the study of fine-scale ecological interactions between local pollinators and their host plants based on insect-focused approaches is hard to develop due to a general lack of curated reference samples. These obstacles restrain our understanding of local wild bees and our capacity to propose well informed conservation initiatives.

In the context of the ‘beelibre’ project (duration 04/2023 – 12/2024) led by the Fondation Faune- Flore (FFF), we propose the development of an open access, user-friendly online database for the storage of national wild bee data, grounding on the results of the “Atlas of the wild bee fauna of Luxembourg” project and the Hymenoptera collection of the MNHNL. This project aims to collate on a single multi-lingual website (beelibre.lu) relevant content concerning wild bee identification, sensibilisation and ecology, building four reference ‘libraries’:

  1. a database of high-quality images taken from live bees and museum specimens (for morpho-taxonomic identification and outreach)
  2. a bibliographic repository of all nationally relevant publications (for metadata analysis and knowledge exchange), incl. short summaries
  3. a pollen inventory pilot experiment aiming to uncover potential ecological interactions between local host flowering plants and their associated wild bee pollinators.
  4. a curated DNA barcode reference library of wild bee species from Luxembourg currently lacking reference material in BOLD systems (for molecular taxonomic identification).

All materials will be freely available to both researchers and the general public, socializing scientific knowledge to a wider audience and raising awareness on national pollinator biodiversity. With this initiative, we aim to provide a space that combines past efforts with current technology, building a platform that can be used to further assist and develop national conservation strategies protecting the wild bee fauna of Luxembourg.

Project status

  • Proposal accepted by the Ministre de l’Environnement, du Climat et du Développement durable in the context of the funding line Appel à projets « Pollinisateurs ». Duration: 20 months (04/2023 – 12/2024).
  • Host institution: Fondation faune-flore @ Centre de recherche scientifique, Musée national d’histoire naturelle.
  • Partners: MECDD

Funding

With financial support from the Fonds pour la protection de l’environnement

Contact

National Museum of Natural History (MNHNL)
Research center
Life science department
25, rue Münster
L-2160 Luxembourg

Publications

 

Generating scientific knowledge, synchronising baseline data and conducting monitoring for subterranean habitats in Luxembourg

Abstract

 

Underground cavities have a high cultural value. As a deposit of prehistoric artifacts and paintings, they provide information about the life of our ancestors and, with their sinter formations, serve as a magnet for tourists and as a climate archive. Yet, the simultaneously high natural value of underground cavities as a structurally rich habitat is often lost.
In Central Europe, around 20 species of bats and about 450 mainly invertebrate animals depend on subterranean terrestrial habitats. The European Union’s Habitats Directive (92/43/EU) recognizes the ecological importance of subterranean sites and protects “caves not open to the public” as a specific habitat type (8310), which implies also a regular reporting to the EU. In Luxembourg, there is no reporting on LRT8310 and no basic status assessment available. Furthermore, semi-natural and artificial underground habitats such as caves, abandoned quarries and mines are recognized and protected as important ecosystems in Luxembourg via the Biotope Protection (BK22). For this reason, the project will

a) provide a basic data collection on the status of LRT8310 and BK22-relevant subterranean habitats in Luxembourg,
b) develop and carry out a representative national monitoring for LRT8310, and
c) scientifically study individual subterranean sites, cave-dwelling organisms and biotopes.

Together, these aspects will produce important scientific findings which will be incorporated into nature and species protection plans at various levels. The data generated will over time lead to a better general understanding of the drivers of the decline in underground biodiversity and serve to develop scientifically sound countermeasures.

Project status

  • Project granted by the Fonds d’environnement of the MECDD. Duration: 6 years (30.09.2020 – 31.06.2026).
  • Host institution: Fondation faune-flore @ Centre de recherche scientifique, Musée national d’histoire naturelle.
  • Partners: MECDD, ANF, Groupe Spéléologique Luxembourgeois (GSL), ProChirop, Dieter Weber & Hannah Weigand

Funding

With financial support from the Fonds pour la protection de l’environnement.

Contact

National Museum of Natural History (MNHNL)
Research center
Life science department
25, rue Münster
L-2160 Luxembourg

Publications

  • Weigand AM, Bücs S-L, Deleva S, Lukić Bilela L, Nyssen P, Paragamian K, Ssymank A, Weigand H, Zakšek V, Zagmajster M, Balázs G, Barjadze S, Bürger K, Burn W, Cailhol D, Decrolière A, Didonna F, Doli A, Drazina T, Dreybrodt J, Ðud L, Egri C, Erhard M, Finžgar S, Fröhlich D, Gartrell G, Gazaryan S, Georges M, Godeau J-F, Grunewald R, Gunn J, Hajenga J, Hofmann P, Knight LRFD, Köble H, Kuharic N, Lüthi C, Munteanu CM, Novak R, Ozols D, Petkovic M, Stoch F, Vogel B, Vukovic I, Hall Weberg M, Zaenker C, Zaenker S, Feit U, Thies J-C (2022) Current cave monitoring practices, their variation and recommendations for future improvement in Europe: A synopsis from the 6th EuroSpeleo Protection Symposium. Research Ideas and Outcomes 8: e85859. https://doi.org/10.3897/rio.8.e85859

Using landscape genetic techniques to understand the impact of habitat fragmentation on the dispersal of insect pollinators (Diptera: Syrphidae)

Abstract

Myathropa floreaPollinators provide a key ecosystem service and their importance for food security is widely acknowledged. Alarmingly though, wild pollinators are declining at local, regional, and global scales, primarily as a consequence of human activities. While land-use change is often seen as a main driver of the problem, there is a considerable lack of knowledge on the responses of invertebrate pollinators to the resulting habitat loss and homogenisation. For instance, while hoverflies (Diptera: Syrphidae) are the most important pollinators besides bees, little is known about their dispersal in general and the effects of landscape fragmentation on their dispersal in particular. The overall objective of the proposed study is therefore to use microsatellite genotyping and landscape genetics to analyse the functional connectivity of typical Luxembourg and Western European landscapes from the viewpoint of hoverflies. We aim to understand whether urbanisation and the structural diversity of agro-ecosystems have an impact on hoverfly dispersal and which landscape features facilitate or hinder gene flow. The approach will be conducted for four target species in three urbanised areas and two pairs of rural areas with different degrees of habitat diversity. Furthermore, we will test whether hoverfly size and habitat specialisation may influence the impact of habitat fragmentation on the flies. Finally, we aim to test the validity of extrapolating connectivity models to larger or different study regions. The proposed project has the potential to make an important contribution in the design of effective habitat management practices for hoverflies, if not insect pollinators in general, since we propose to empirically test dispersal capabilities in different landscapes, taking different biological and ecological characteristics into account.

Project status

  • Project granted by the granted by Fonds National de la Recherche Luxembourg. Duration: 3 years (01.02.2021 – 31.01.2024).
  • Host institution: Fondation faune-flore @ Centre de recherche scientifique, Musée national d’histoire naturelle.
  • Postdoctoral Researcher to be hired.

Contact

National Museum of Natural History (MNHNL)
Research centre
Life science department
25, rue Münster
L-2160 Luxembourg

Publications

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Using genetic methods to understand the movement ecology of the crested newt (Triturus cristatus) in Luxembourg

Abstract

The Crested Newt (Triturus cristatus); male specimen in "mating dress" under water.Populations of the pond-breeding Northern crested newt (Triturus cristatus) have severely declined in Luxembourg during the last century. The species is listed on Annexes II and IV of the EU Habitats Directive (Council of the European Communities, 1992) and EU member states must therefore ensure the maintenance or, where appropriate, the re-establishment of a favourable state of conservation of the species’ and its habitats. In order to maintain the species’ metapopulation dynamics, more than 350 artificial freshwater bodies have been created in the west and southwest of the country since 1993. This conservation measure lacked a certain degree of effectiveness as fewer than 75% of the engineered ponds have been colonized by the target species. There is thus an urgent need to generate an evidence-based understanding of the movement ecology of crested newts in the Luxembourg context and to design and place engineered ponds appropriately. We present three work packages that will operate at different spatial scales and that will use population and landscape genetic methods to identify landscape factors influencing newt dispersal and population connectivity. Specifically, we will i) analyse the population genetic structure of the crested newt across Luxembourg in order to identify isolated populations and environmental factors that hinder exchange between populations, ii) model landscape connectivity at the regional scale to empirically identify landscape features that impact on exchange between ponds and to predict crested newt movements across the landscape, iii) identify optimal locations for new ponds using a systematic conservation planning (site-selection algorithm) approach. The overriding objective of the proposed research project is to identify efficient conservation measures that will ensure the long-term survival of crested newts in Luxembourg.

Project status

  • Project granted by the Ministry of Environment, Climate and Sustainable Development (MECDD). Duration: 18 months (01.10.2019 – 31.03.2021).
  • Host institution: Fondation faune-flore @ Centre de recherche scientifique, Musée national d’histoire naturelle.

Contact

National Museum of Natural History (MNHNL)
Research centre
Life science department
24, rue Münster
L-2160 Luxembourg

Publications

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MosquitoLUX – Atlas of the mosquitoes of Luxembourg

Abstract

Culex modestus, first detected in June 2019 in Remerschen.

The project MosquitoLUX (2019-2022) aims to compile and complete knowledge on mosquitoes (Diptera: Culicidae) in Luxembourg. In a context of climate and environmental change, this first mosquito atlas should make it possible to draw up an inventory of Culicidae fauna, in terms of biodiversity first, but also in terms of public health and vector risks. The latter requires not only the collection of presence/absence data, but also abundance and seasonality according to different environments. This inventory should also make it possible to detect introduced invasive alien species and changes in the distribution areas of native species.

Website: https://mosquitoes.lu/

Main researchers

  • Dr Francis Schaffner, expert in medical and veterinary entomology, Francis Schaffner Consultancy (Surveillance and management of biting insects), Riehen, Switzerland.
  • Dr Christian Ries, curator, Department of Ecology, Luxembourg National Museum of Natural History.
  • Dr Alexander Weigand, curator, Department of Zoology, Luxembourg National Museum of Natural History.

Project status

Funding consortium :

  • 50% National Museum of Natural History (MNHNL). Involved departments: Ecology and Zoology.
  • 25% Ministry of Environment, Climate and Sustainable Development (MECDD).
  • 25% Ministry / Directorate of Health (M/DS).

Duration: 2 years (01.04.2019 – 31.03.2021). Due to the Covid-19 pandemic, field surveys were delayed and completed in 2022. The manuscript has been published in January 2023:

Schaffner F., Weigand A., Ries C. Atlas and catalogue of the mosquitoes (Diptera, Culicidae) of Luxembourg Ferrantia 87, Musée national d’histoire naturelle, Luxembourg, 117 p.

Host institution and administrative leader: Fondation faune-flore @ Centre de recherche scientifique, Musée national d’histoire naturelle.

Contact

Christian RIES
Musée national d’histoire naturelle
Research centre
Life science department
Research units: Ecology
24, rue Münster
L-2160 Luxembourg

Publications

  • Schaffner, F., Weigand, A. & C. Ries, 2023. Atlas and catalogue of the mosquitoes (Diptera, Culicidae) of Luxembourg. Ferrantia 87, Musée national d’histoire naturelle Luxembourg, 117 p. [PDF 22,25 MB]
  • Schaffner, F. & C. Ries, 2019. First evidence and distribution of the invasive alien mosquito Aedes japonicus (Theobald, 1901) in Luxembourg. Bull. Soc. Nat. luxemb. 121: 169-183. [PDF 8,94 MB]

Genetic monitoring of the pool frog and the edible frog in Luxembourg

Abstract

Nowadays, most animal species can be identified genetically by sequencing a fragment of the mitochondrial barcoding gene COI. However, for some species, this method is not applicable due to a more complex genetic make-up, often due to hybridisation.

One of these exceptions are the green frogs (pool frog – Pelophylax lessonae, edible frog – P. kl. esculentus and marsh frog – P. ridibundus). This group of frogs has an especially complex genetic composition due to hemiclonal hybridisation and polyploidisation.

To establish a genetic monitoring tool-kit for green frogs in Luxembourg, we will first apply ddRAD sequencing to a wide range of populations from the whole country. This high-throughput sequencing based method will be used to generate hundreds of genome-wide genetic markers. The resulting dataset will allow to identify individuals on species level and to assess their ploidy. Furthermore, it will enable an estimation of the genetic diversity of the two common species in Luxembourg, the pool frog and the edible frog.

In a second step of the project, these genetic data will be used to establish genetic markers for standard genetic monitoring in Luxembourg and to develop recommendation guidelines for their application.

Foto Frosch
Pelophylax sp. (water frog) – Peppange – 09.05.2018 © Hannah Weigand.

Project status

Project granted by the Ministry of Sustainable Development and Infrastructure, Luxembourg. Duration: one year (15.04.2018 – 14.04.2019).

Host institution: Fondation faune-flore @ Centre de recherche scientifique, Musée national d’histoire naturelle.

Contact

Hannah Weigand
Musée national d’histoire naturelle
Centre de recherche scientifique
Zoologie des vertébrés
24, rue Münster
L-2160 Luxembourg
phone: (+352) 462240 212

Publications

  • WEIGAND H. & Leese F. (2018) Detecting signatures of positive selection in non-model species using genomic data. Zoological Journal of the Linnean Society.
  • WEIGAND H., Weiss M., Cai H., Li Y., Yu L., Zhang C., Leese F. (2018) Fishing in troubled waters: Revealing genomic signatures of local adaptation in response to freshwater pollutants in two macroinvertebrates. Science of the total environment 633:875-891.
  • Weiss M., WEIGAND H., Weigand A.M., Leese F. (2018) Genome-wide SNP data reveal cryptic species
    within cryptic freshwater snail species – The case of the Ancylus fluviatilis species complex. Ecology
    and Evolution 8:1063-1072.
  • Steinbach P., Heddergott M., WEIGAND H., Weigand A.M., Wilwert E., Stubbe M., Helm B., Campbell R.E., Stubbe A., Frantz A.C. (2018) Rare migrants suffice to maintain high genetic diversity in an introduced island population of roe deer (Capreolus capreolus): evidence from molecular data and simulations. Mammalian Biology 88:64-71.
  • WEIGAND H., Weiss M., Cai H., Li Y., Yu L., Zhang C., Leese, F (2017) Deciphering the origin of mito-nuclear discordance in two sibling caddisfly species. Molecular Ecology 26:5705-5715.
  • Vendrami D.L.J., Telesca L., WEIGAND H., Weiss M., Fawcett K., Lehman K., Clark M.S., Leese F., McMinn C., Moore H., Hoffman J.I. (2017) RAD sequencing resolves fine-scale population structure in a benthic invertebrate: implications for understanding phenotypic plasticity. Royal Society open science 4:160548.
  • SCHWEYEN H., Rozenberg A., Leese F. (2014) Detection and removal of PCR duplicates in population genomic ddRAD studies by yddition of a degenerate base region (DBR) in sequencing adapters. Biological Bulletin 227:146-160.
  • Magwire M.M.*, Fabian D.K.*, SCHWEYEN H.*, Cao C., Longdon B., Bayer F., Jiggins F.M. (2012) Genome-wide association studies reveal a simple genetic basis of resistance to naturally coevolving viruses in Drosophila melanogaster. PLOS Genetics 8:e1003057 (* equally contributing authors).
  • Scharsack J.P., SCHWEYEN H., Schmidt A.M., Dittmar J., Reusch T.B.H., Kurtz J. (2012) Population genetic dynamics of three-spined sticklebacks (Gasterosteus aculeatus) in anthropogenic altered habitats. Ecology and Evolution 2:1122-1143.

DNADIVE

Full title

DNADIVE: eDNA Detection for crayfish invasIVE species in streams of Luxembourg: Development of a molecular toolbox


This public-public research project is supported by the
Luxembourg National Research Fund (PUBLIC2-17/SR/11816664)

Abstract

Oronectes_limosusDNADIVE aims at developing a toolbox for the molecular monitoring of invasive crayfish in the streams of Luxembourg. Three exotic species (Orconectes limosus, Pacifastacus leniusculus and Astacus leptodactylus) and a native one (Astacus astacus) will be targeted during this project. This molecular toolbox will encompass several techniques of detection comprising (1) a simple amplification method easily performed in a laboratory with few elements, (2) a digital droplet amplification (ddPCR) which is a more elaborated lab method that can allow for a higher detection sensitivity and a possible quantification of DNA that could be related through the proxy of biomass and abundance to the size of the populations detected and (3) an isotherm amplification method (iPCR) i.e. a simple, cost effective approach which will allow for a field detection usable by non-trained agents. The results will enable the development of a predictive species distribution model for the target species and allow to infer their impact on freshwater communities through the comparison with previous sampling campaigns.

This set of methods has the high potential to efficiently contribute to early detection and routine monitoring of the invasive crayfish species in Luxembourg, thus allowing for a timely and efficient decision-making and appropriate management.

Project status

Project funded by Fonds national de la recherche Luxembourg, Musée national d´histoire naturelle Luxembourg, Ministry of sustainable development and infrastructure. Duration: 3 years (01.05.2018 – 30.04.2021).

Project consortium: Fondation faune-flore, Musée national d´histoire naturelle Luxembourg, Luxembourg Institute of Science and Technology, Ministry of sustainable development and infrastructure.

Host institution: Fondation faune-flore @ Centre de recherche scientifique, Musée national d’histoire naturelle.

Contact

David PORCO
Musée national d’histoire naturelle
Research centre
Life science department
Research units: Invertebrate zoology & Population biology and Evolution
24, rue Münster
L-2160 Luxembourg
phone: (+352) 46 22 33 -264
fax: (+352) 46 38 48

Publications

Molecular taxonomy (DNA barcoding)

  • PORCO D., Chang C., Dupont L., James S., Richard B., Decaëns T. (2017) A reference library of DNA barcodes for the earthworms from Upper Normandy: biodiversity assessment, new records, potential cases of cryptic diversity and ongoing speciation. Applied Soil Ecology – Sous presse.
  • Potapov M., PORCO D., Deharveng L. (2017) A new member of the genus Isotomurus from Kuril Islands (Collembola: Isotomidae) : returning to the problem of “color pattern species“. Zootaxa – Soumis.
  • Shekhovtsov S.V., Berman D.I., Bazarova N.E., Bulakhova N.A., PORCO D., Peltek S.E. (2016) Cryptic genetic lineages in Eisenia nordenskioldi pallida (Oligochaeta, Lumbricidae) and diversification history of the E. nordenskioldi complex. European Journal of Soil Biology, 75:151-156.
  • Dupont L., PORCO D., Symondson W.O.C., Roy V. (2016) Hybridization relics complicate barcode-based identification of species in earthworms. Molecular Ecology Ressources, 16: 883-894.
  • Cameron E.K., Decaëns T., Lapied E., PORCO D., Eisenhauer N. (2016) Earthworm databases and ecological theory: Synthesis of current initiatives and main research directions. Applied Soil Ecology, 104: 85-90.
  • Paoletti M.G., Blakemore R.J., Csuzdi C., Dorigo L., Dreon A.L., Gavinelli F., Lazzarini F., Manno N., Moretto E., PORCO D., Ruzzier E., Toniello V., Squartini A., Concheri G., Zanardo M., Alba-Tercedor J. (2016) Barcoding Eophila crodabepis sp. nov. (Annelida, Oligochaeta, Lumbricidae), a large stripy earthworm from Alpine foothills of Northeastern Italy similar to Eophila tellinii (Rosa, 1888). PLoS One, 11: e0151799.
  • Schneider C., PORCO D., Deharveng L. (2016) Two new Megalothorax species of the minimus group (Collembola, Neelidae). ZooKeys, 554: 37-68.
  • Decaëns T., PORCO D., James S.W., Brown G.G., Chassany V., Dubs F., Dupont L., Lapied E., Rougerie R., Rossi J.-P., Roy V. (2016) DNA barcoding reveals diversity patterns of earthworm communities in remote tropical forests of French Guiana. Soil Biology and Biochemistry, 92: 171-183.
  • Lukić M., PORCO D., Bedos A., Deharveng L. (2015) The puzzling distribution of Heteromurus (Verhoeffiella) absoloni Kseneman, 1938 (Collembola: Entomobryidae: Heteromurinae) resolved: detailed redescription of the nominal species and description of a new species from Catalonia (Spain). Zootaxa, 4039: 249-275.
  • Deharveng L., Zoughailech A., Hamra-Kroua S., PORCO D. (2015) A new species of Deutonura (Collembola: Neanuridae: Neanurinae) from north-eastern Algeria, and characterisation of two intraspecific lineages by their barcodes. Zootaxa, 3920, 281-290.
  • PORCO D., Skarżyński D., Decaëns T., Hebert P.D.N., Deharveng L. (2014) Barcoding the Collembola of Churchill: a molecular taxonomic reassessment of species diversity in a sub-Arctic area. Molecular Ecology Ressources, 14, 249-261.
  • Greenslade P., Stevens M.I., PORCO D. (2013) Measuring changes in molecular and geographical distribution after forty years of a possible endemic genus of South Australian Collembola (Springtail). The South Australian Naturalist 87, 18-21.
  • PORCO D., Decaëns T., Deharveng L., James S.W., Skarżyński D., Erséus C., Butt K., Richard B., Hebert P. (2013) Biological invasions in soil: DNA barcoding as a monitoring tool in a multiple taxa survey targeting European earthworms and collembolans in North America. Biological Invasions 15: 899-910.
  • Stoev, P., Komerički, A., Akkari, N., Liu, S., Zhou, X., Weigand, A., Hostens. J., Hunter C., Edmunds S., PORCO D., Zapparoli M., Georgiev T., Mietchen D., Roberts D., Faulwetter S., Smith V., Penev, L. (2013) Eupolybothrus cavernicolus Komerički & Stoev sp. n. (Chilopoda: Lithobiomorpha: Lithobiidae): the first eukaryotic species description combining transcriptomic, DNA barcoding and micro-CT imaging data. Biodiversity Data Journal, 1, e1013.
  • Decaëns T., PORCO D., Rougerie R., Brown G.G., James S.W. (2013) Potential of DNA barcoding for earthworm research in taxonomy and ecology. Applied Soil Ecology 65: 35-42.
  • PORCO D., Potapov M., Bedos A., Busmachiu G., Weiner W.M., Hamra-Kroua S., Deharveng L. (2012) Cryptic diversity in the ubiquist species Parisotoma notabilis (Collembola, Isotomidae): a long used chimeric species? PLoS One 7: e46056.
  • PORCO D., Bedos A., Greenslade P., Janion C., Skarżyński D., Stevens M.I., Jansen van Vuuren B., Deharveng L. (2012) Challenging species delimitation in Collembola: cryptic diversity among common springtails unveiled by DNA barcoding. Invertebrates Systematics 26: 470-477.
  • Dózsa-Farkas K., PORCO D. and Boros G. (2012) Are Bryodrilus parvus Nurminen, 1970 and Bryodrilus librus (Nielsen and Christensen, 1959) (Annelida: Enchytraeidae) really different species? A revision based on DNA barcodes and morphological data. Zootaxa 3276: 38-50.
  • Janion C., Bedos A., Bengtsson J., Deharveng L., Jansen van Vuuren B., Leinaas H. P., Liua A., Malmström A., PORCO D. and Chown Steven L. (2011) Springtail diversity in South Africa. South African Journal of Science 107: 75-81.
  • Stevens M.I., PORCO D., D’Haese C.A. and Deharveng L. (2011) Comments on “Taxonomy and the DNA Barcoding Enterprise” by Ebach (2011). Zootaxa 2838: 85-88.
  • Dupont L., Lazrek F., PORCO D., King R.A., Rougerie R., Symondson W.O.C., Livet A., Richard B., Decaëns T., Butt K. R. and Mathieu J. (2011) New insight into the genetic structure of the Allolobophora chlorotica aggregate in Europe using microsatellite and mitochondrial data. Pedobiologia 54: 217-224.
  • James S.W., PORCO D., Decaëns T., Richard B., Rougerie R. and Erséus C. (2010) DNA barcoding reveals cryptic diversity in Lumbricus terrestris L., 1758 (Clitellata): Resurrection of L. herculeus (Savigny, 1826). PLoS One 5: e15629.
    PORCO D., Bedos A. and Deharveng L. (2010) Description and DNA barcoding assessment of the new species Deutonura gibbosa (Collembola: Neanuridae: Neanurinae), a common springtail of Alps and Jura. Zootaxa, 2639: 59-68.
  • PORCO D., Rougerie R., Deharveng L. and Hebert P. (2010) Coupling non-destructive DNA extraction and voucher retrieval for small soft-bodied Arthropods in a highthroughput context: the example of Collembola. Molecular Ecology Resources, 10: 942-945.
  • Stoev P., Akkari N., Zapparoli M., PORCO D., Enghof H., Edgecombe G. D., Georgiev T. and Penev L. (2010) The centipede genus Eupolybothrus Verhoeff, 1907 (Chilopoda: Lithobiomorpha: Lithobiidae) in North Africa, a cybertaxonomic revision, with a key to all species in the genus and the first use of DNA barcoding for the group. ZooKeys, 50: 29-77.
  • Richard B., Decaëns T., Rougerie R., James S., PORCO D., Hebert P. (2010) Re-integrating earthworm juveniles in soil diversity studies: species identification through DNA barcoding. Molecular Ecology Resources, 10: 606–614.
  • Rougerie R., Decaëns T., Deharveng L., PORCO D., James S. W., Chang C., Richard B. and Hebert P. D.N. (2009) DNA barcodes for soil animal taxonomy: transcending the final frontier. Pesquisa Agropecuaria Brasileira, 44: 789-802.

Chemical ecology

  • PORCO D., Bedos A. and Deharveng L. (2010) Cuticular compounds bring new insight in the post-glacial recolonization of a Pyrenean area: Deutonura deficiens Deharveng, 1979 complex, a case study. PLoS One 5: e14405.
  • PORCO D. and Deharveng L. (2009) Phylogeny of Collembola based on cuticular compounds: inherent usefulness and limitation of a character type. Naturwissenschaften, 96: 943-954.
  • PORCO D., Deharveng L. and Skarżyński D. (2009) Sex pheromone in Tetrodontophora bielanesis (Waga, 1842) (Collembola: Onychiuridae): Indirect reproduction mediated by cuticular compounds. Pedobiologia, 53: 59-63.
  • PORCO D. and Deharveng L. (2007) 1,3-dimethoxybenzene, a chemotaxonomic marker for the Neanurinae subfamily (Collembola). Biochemical Systematics and Ecology, 35: 160-161.
  • PORCO D., Deharveng L. and Gers C. (2004) Sexual discrimination with cuticular lipids in Schoetella ununguiculata (Collembola). Pedobiologia, 48: 581-583.

Hybrid zones

  • Rougerie R., Deharveng L., PORCO D. and Bedos A. (2006) Geographical barriers and ecological gradients: geographical distribution of the subspecies and morphotypes of Deutonura deficiens Deharveng 1979 (Collembola: Neanuridae). Pedobiologia, 50: 83-93.

Climate change, plasticity and genetic variation in Anthyllis vulneraria

Full title

Climate change, plasticity and genetic variation in Anthyllis vulneraria

Abstract

Climate is a key factor determining plant distribution. Predictions of rapid climate change have resulted in a renewed interest in the possible responses of plants to changes in climate, such as migration to other localities, phenotypic plasticity and local adaptation. The aim of this project is to study the possible impact of predicted climate change on the short-lived perennial plant Anthyllis vulneraria along environmental gradients. We will analyze natural populations and their habitats along altitudinal and latitudinal gradients to investigate relationships between population characteristics and environmental conditions. We will also investigate molecular genetic diversity in relation to the same gradients. The variation in a number of quantitative traits and its distribution among spatial scales will be studied in a lowland common garden and compared with that of the variation in neutral genetic markers.

We will test whether plants from mountain and high latitude populations perform less well under lowland conditions than plants from lowland and central European populations, and whether genotypes exist in the mountain and high latitude populations that are preadapted to the predicted warmer future conditions. Plants will be genotyped to detect loci correlated with ecological gradients which are of ecological relevance in A. vulneraria.

Predicted higher temperatures are likely to increase the frequency of drought. To assess the role of plasticity and evolutionary adaptation in the response to increased droughts, both the plastic responses of high latitude / altitude and lowland populations to drought and genetic variation in drought resistance will be studied experimentally. The expected results will contribute to a better understanding of the role of plasticity, genetic diversity and local adaptation of short-lived grassland plants under predicted climate change. The outcomes will also have implications for the conservation and management of biodiversity.

Status of Phd

Supervisor at university: Prof. Dr. Diethart Matthies, Plant ecology, University of Marburg

Supervisor in Luxembourg: Dr. Guy Colling, institution : Scientific Research Centre of the National Museum of Natural History

PhD granted by Fonds national de la recherche Luxembourg. Duration: 3 years (01.09.2014 – 30.08.2017, 01.09.2017 – 30.08.2018).

Host institution: Fondation faune-flore @ Centre de recherche scientifique, Musée national d’histoire naturelle.

Contact

Laura Daco
Musée national d’histoire naturelle
Centre de recherche scientifique
Biologie des populations et évolution
24, rue Münster
L-2160 Luxembourg
phone: (+352) 462240 -265
fax: (+352) 46 38 48

Publications

  • Daco, L., Matthies, D., Hermant, S., & Colling, G. (2022). Genetic diversity and differentiation of populations of Anthyllis vulneraria along elevational and latitudinal gradients. Ecology and Evolution, 12(8). https://doi.org/10.1002/ece3.9167
  • Daco, L., Colling, G., & Matthies D. (2021) Altitude and latitude have different effects on population characteristics of the widespread plant Anthyllis vulneraria. Oecologia, 197, 1-13. https://doi.org/10.1007/s00442-021-05030-6
  • Daco, L., T. Maurice, S. Muller, J. Rossa, G. Colling, (2019) Genetic status of the endangered plant species Gladiolus palustris in the western part of its distribution area. Conservation Genetics. https://doi.org/10.1007/s10592-019-01213-0
  • Daco L (2011) Etude phylogénétique et phylogéographique de Gladiolus palustris et Gladiolus imbricatus à l’aide de marqueurs nucléaires ITS. Master thesis, University of Strasbourg, France.

Evolutionary potential of the raccoon roundworm

Full title

Using population genetics to understand the evolutionary potential of an introduced parasitic nematode, the raccoon roundworm (Baylisascaris procyonis)

Abstract

Parasitic nematodes can cause serious diseases and their emergence in new areas can be an issue of major concern for human health, agricultural productivity and biodiversity conservation. Molecular studies of nematode invasions can make a significant contribution to a better understanding of the underlying mechanism of the emergence, spread and persistence of the parasites. Also, they will clarify the evolutionary potential of the invaders in their new range and help with the elaboration of adequate management strategies against helminthic disease. The raccoon roundworm (Baylisascaris procyonis) is a common gastrointestinal parasite of the raccoon (Procyon lotor), that is emerging as an important helminthic zoonoses that mainly affects young children. Translocations of raccoons have increased the global geographic range of its roundworm as well. Raccoons are particularly abundant and wide-spread in Germany, where they can occur at high densities in urban areas. Recent genetic work has provided evidence for at least four independent introduction events, which gave rise to genetically differentiated subpopulations in incipient contact of relatively high genetic diversity. The roundworm, however, does not occur in all raccoon populations and might therefore be much less genetically diverse due to reduced propagule pressure. The overall objective of this study is to use population genetic techniques to analyse the genetic diversity and gene flow in the invasive nematode B. procyonis to understand the evolutionary potential of the parasite and allow the implementation of successful control programmes. We aim in particular to establish how frequently the parasite was introduced into Western Europe, to estimate the geographic origin of the introduced parasites, to examine the changes in genetic diversity during the invasion process and to the landscape genetic structure of the raccoon and its roundworm in parallel.

Status of Phd

Supervisor at university: Prof. Dr. Danuta Kosik-Bogacka, Department of Biology and Medical Parasitology, Pomeranian Medical University, Powstancow Wielkopolskich 72, 70-111 Szczecin, Poland.

Supervisor in Luxembourg: Dr. Alain Frantz, Scientific Research Centre of the National Museum of Natural History, Luxembourg.

Postdoc granted by Fonds national de la recherche Luxembourg. Duration: 2 years (01.11.2014 – 31.10.2016).

Host institution: Fondation faune-flore @ Centre de recherche scientifique, Musée national d’histoire naturelle.

Contact

Natalia Osten-Sacken
Musée national d’histoire naturelle
Centre de recherche scientifique
Laboratoire/Section de zoologie des invertébrés
24, rue Münster
L-2160 Luxembourg
phone: (+352) 46 22 40 -210
fax: (+352) 46 38 48

Publications and not published works

  • Danków R., Pikul J., Lasik A., Teichert J., OSTEN_SACKEN N., 2010. The functional properties and microbiological quality of koumiss liophilisates. Probiotyki w żywności. Polskie Towarzystwo Technologów Żywności, Wydawnictwo Naukowe PTTŻ: 237- 251.
  • OSTEN-SACKEN, N. Rychlik L., 2011. Diät vom Marderhund (Nyctereutes procyonoides) in Westpolen- erste Ergebnisse. Beiträge zur Jagd und Wildforschung, Bd. 36: 171-180.
  • OSTEN-SACKEN, N. Rychlik L., 2011, Erste Ergebnisse radiotelemetrischer Untersuchungen zur Raumnutzung von Marderhunden (Nyctereutes procyonoides) in Westpolen. Beiträge zur Jagd und Wildforschung, Bd. 36: 181-189.
  • OSTEN-SACKEN N., Ziomek J. Kardynia P., Zgrabczyńska E., 2011. Distribution of the raccoon dog (Nyctereutes procyonoides) in western Poland. Fragmenta Faunistica 54 (1) 95-102.
  • Danków R., Pikul J., OSTEN-SACKEN N., Teichert J., 2012. Characteristics and salubrious properties of mare milk. Nauka Przyroda Technologie, 6 (2):1-12, www. npt.up-poznan.net.
  • Danków R., Pikul J., Teichert J., OSTEN-SACKEN N., 2013. Characteristics and properties of koumiss. Nauka Przyroda Technologie, 7, (3)#35:1-16. www. npt.up-poznan.net.
  • Danków R., Teichert J., Pikul J., OSTEN-SACKEN N., 2013. Characteristics of dietary and quality attributes of mare milk lyophilisates. Nauka Przyroda Technologie, 7, (4) #63: 1-10. www. npt.up-poznan.net
  • Danków R., Teichert J., Pikul J.,OSTEN-SACKEN N., 2013. Properties of fermented beverages from modified cow᾽s milk. Nauka Przyroda Technologie, 7 (4) #70 :1-9. www. npt.up-poznan.net.
  • Nowosad P., OSTEN-SACKEN N., Solarczyk P., 2013, Trichinella spiralis in road- killed raccoon dogs (Nyctereutes procyonoides). Annals of parasitology, Polish Parasitological Society, Volume 59, page 51.
  • Słodkowicz Kowalska A., Pacoń J., Skrzypczak Ł., Werner A., OSTEN_SACKEN N.,Majewska A.C., 2013. Intestinal parasites of raccoon dog (Nyctereutes procyonoides) from western Poland. Annals of parasitology, Polish Parasitological Society, Volume 59, page 58.

Popular articles

  • OSTEN-SACKEN N. (2002): Slots. The hidden abilities. Magazine of Life Sciences Salamandra Biuletyn PTOP Salamandra I-II (16): 35-36.
  • OSTEN-SACKEN N., Ziomek J. (2004): Raccoon dog – a visitor from the Far East. Magazine of Life Sciences Salamandra I(18): 17-18.
  • OSTEN-SACKEN N. (2005): The European Union and youth projects. Magazine of Life Sciences Salamandra I(20): 36-37.
  • OSTEN-SACKEN N., Ziomek J. (2005): How our Western neighbors protect otters. Magazine of Life Sciences SalamandraII(21): 20-24.

Talks and poster presentations

  • OSTEN-SACKEN N., Pastok D. and Rychlik L, 2008, The raccon dog and the raccoon as threats for the native fauna in western Poland – the study project and first results on the prey fauna [In: Abstracts book Protection of free-living mammal populations:20-21], 26-28.09., Zaklad Zoologii Systematycznej, Biologia Srodowiska UAM, Poznan, Poland.
  • OSTEN-SACKEN N. and Rychlik L.,  2008, Marderhund und Waschbär als Potentielle Gefahr für die einheimische Fauna – Einführungsuntersuchungen [In: Abstracts book Einwandernde Tierarten-wie sind wir darauf vorbereitet:7-8], 16-17.10., Branderburgische Akademie Schloss Criewen, Germany [in German].
  • OSTEN-SACKEN N., Rychlik L., 2008, Cirkadian activity of the small mammals in the „Ujscie Warty“ National Park. Poster. Scientific session: behavioral sciences in Nencki Institut- traditions, present and future, 24.11., Nencki Institut of Experimental Biology, Warsaw, Poland.
  • OSTEN-SACKEN N., Rychlik L., 2009, Differentiation of habitat and temporal niches among small mammals co-existing in the National Park “Ujscie Warty” western Poland, [in: Abstract Book :59], 5th “Ecology and Behaviour” Meeting, 6-10 April, Lyon, France.
  • OSTEN-SACKEN, N., 2009, Parasitenbefall von Marderhunden im Nationalpark Warthemündung im Vergleich zu anderen polnischen Gebieten. Internationales Symposium der Gesellschaft für Wildtier und Jagdforschung „Wildhygiene, Wildtierkrankheiten, Parasiten, Epidemiologie“, 16-19 April, Friedrichsbrunn, Germany [in German].
  • OSTEN-SACKEN N., Rychlik, L., 2009, Is the invasive mammal raccoon dog Nyctereutes procyonoides the bigger threat for the native fauna than the native predators? [in: Abstract Book :111], poster. World Conference on Biological Invasions and Ecosystem Functioning, 27-30 October, Porto, Portugal.
  • OSTEN-SACKEN N., Górska K., Rychlik L., 2011, Diet of the raccoon dog (Nyctereutes procyonoides) in western Poland – preliminary results, [in; Abstract book Konferencja Naukowo-Dydaktyczna Wydzialu Biologii. Wyzwania wspólczesnej biologii, biotechnologii i ochrony srodowiska:76], poster., Adam Mickiewicz University 5-7 April.
  • OSTEN-SACKEN N., Bednarz P., Rychlik L., 2011, Diet of the stone marten (Martes foina) in western and northern Poland,  [in; Abstract book Konferencja Naukowo-Dydaktyczna Wydzialu Biologii. Wyzwania wspólczesnej biologii, biotechnologii i ochrony srodowiska:62], poster., Adam Mickiewicz University 5-7 April.
  • OSTEN-SACKEN N., Rychlik L., 2011, Radiotelemetrische Untersuchung zur Raumnutzung des Marderhundes (Nyctereutes procyonoides) in Westpolen, Intenationales Symposium (20 Jahre Gesellschaft für Wildtier und Jagdforschung, PopulationsÖkologie von Raubsäugerarten, Camp Reinsehlen, April 7-10 ,Germany.
  • OSTEN-SACKEN N., Rychlik L., 2011, Diät des Marderhundes (Nyctereutes procyonoides) in Westpolen, poster. Intenationales Symposium (20 Jahre Gesellschaft für Wildtier und Jagdforschung, PopulationsÖkologie von Raubsäugerarten, Camp Reinsehlen, April 7-10 ,Germany.
  • OSTEN-SACKEN N, Rychlik L., 2011, Habitat Using, home range and activity patterns of the invasive mammal raccoon dog (Nyctereutes procyonoides) in western Poland [in Abstract book: 23] 7th Ecology and Behaviour meeting , Rennes, May 2-6, France.
  • OSTEN-SACKEN, Górska K, Rychlik L., 2011, Composition of the food of stone marten (Martes foina) in Gorzów Wielkopolski, Poland and Slowinski National Park, Poland, [in Abstract book: 94], poster. 7th Ecology and Behaviour meeting , Rennes, May 2-6, France.
  • OSTEN-SACKEN, Górska K., Rychlik L., 2011, Food composition of the raccoon dog (Nyctereutes procyonoides) in western Poland – preliminary results, [in Abstract book:102] poster. 7th Ecology and Behaviour meeting , Rennes, May 2-6, France.
  • Danków R, Pikul, J, OSTEN-SACKEN N., 2011, Effect of lactation on the some milk physicochemical traits of polish cold blood breed mares, poster. IDF International Symposium on sheep, goat and other non -cow milk. 16-18 may, Athens, Greece.
  • Danków R, Pikul, J, OSTEN-SACKEN N., 2011. Characteristics of koumiss made from mares milk of the Wielkopolski breed, poster. IDF International Symposium on sheep, goat and other non -cow milk. 16-18 may, Athens, Greece
  • OSTEN-SACKEN N., 2012, Marderhund in Polen: Bedrohung für die einheimische Fauna? Untersuchungen und Ergebnisse. 9. Fachtagung Jagd und Artenschutz, 9-10 March, Jena, Germany.
  • OSTEN-SACKEN N., Rychlik L., 2013, Diet of the raccon dog (Nyctereutes procyonoides ) from western Poland, based on stomach contents and feces analyses, [in: Abstract book: 95]. 11th International Mammalogical Congress, Queens University of Belfast ,11-16 August.
  • OSTEN-SACKEN N, Rychlik L., 2013, Parasites from feces and intestines of the raccoon dog  (Nyctereutes procyonoides), including species dangerous for people, [in: Abstract book: 137] 11th international Mammalogical Congress, Queens University of Belfast, 11-16 August.
  • Slodkowicz- Kowalska A., Pacon J., Skrzypczak L., Werner A., OSTEN-SACKEN N., 2013, The intestines parasites of the raccoon dog (Nyctereutes procyonides) from western Poland. The XXIIIth Congress of the Polish Parasitological Society, 4-7 September, Szklarska Poreba –Piechowice, Poland.
  • Nowosad P., OSTEN-SACKEN N., Solarczyk P., 2013, Trichinella spiralis by road-killed raccoon dogs (Nyctereutes procyonoides),  The XXIIIth Congress of the Polish Parasitological Society, 4-7 September, Szklarska Poreba –Piechowice, Poland.